In Vitro Antimicrobial Activity Analysis of Green Synthesized Copper Nanoparticles with Amaranthus dubius Leaf Extract Against Bacillus subtilis & Salmonella typhi (KEY IJP************818)

• Dr. Rupak Roy

Abstract

The present study explores a green and eco-friendly approach for the synthesis of copper nanoparticles (CuNPs) using Amaranthus dubius leaf extract and evaluates their antimicrobial efficacy against Bacillus subtilis and Salmonella typhi. The extracellular biogenic synthesis of CuNPs using A. dubius is a novel approach, as this plant has not been previously employed for nanoparticle synthesis. Phytochemicals in the leaf extract facilitated the reduction of Cu ions, leading to the formation of well-defined CuNPs. The synthesized CuNPs were tested for their antimicrobial activity using the agar well diffusion method. The results demonstrated that S. typhi exhibited higher susceptibility to CuNPs, showing a prominent zone of inhibition across all tested concentrations, whereas B. subtilis displayed resistance at lower concentrations but showed significant inhibition at higher CuNP concentrations. This study confirms the potential of green-synthesized CuNPs as effective antimicrobial agents, highlighting their promising applications in pharmaceuticals, biomedical research, and nanotechnology-based therapeutics.